anti p nf κb p50 (Santa Cruz Biotechnology)

Santa Cruz Biotechnology is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology anti p nf κb p50

Anti P Nf κb P50, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 191 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nf+%CE%BAb+p50/p-NF%CE%BAB+p50+Antibody/pmc13657245-69-0-5
Average 93 stars, based on 191 article reviews

anti p nf κb p50 - by Bioz Stars, 2026-10

93/100 stars

Images

1) Product Images from "M2 type tumor-associated macrophage-derived CCL-18 promotes glioma cell proliferation but does not mediates glioma recurrence after radiotherapy"

Article Title: M2 type tumor-associated macrophage-derived CCL-18 promotes glioma cell proliferation but does not mediates glioma recurrence after radiotherapy

Journal: iScience

doi: 10.1016/j.isci.2026.117695

Figure Legend Snippet:

Techniques Used: Cell-Signaling, Virus, Tissue, Microarray, Recombinant, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Software, Biomarker Discovery, In Vivo Imaging

Blocking Assay:

Article Title: Anti‐Cancer Effect of Sulforaphane in Human Pancreatic Cancer Cells Mia PaCa ‐2
Article Snippet: Then, it was transferred to 0.45 μm Immobilon‐P transfer membranes (Merk Millipore, Darmstadt, Germany). .. After transfer, it was blocked in a 2.5% BSA blocking buffer for 1 h. After blocking, primary antibodies such as anti‐glycogen synthase kinase‐3 beta (GSK‐3β; #5558, 1:1000, Cell Signaling Technology, Beverly, MA, USA), phosphorylated (p‐)GSK‐3β (Ser9; #12456, 1:1000, Cell Signaling), phosphorylated (p‐)NF‐κB p65 (#3033, 1:1000, Cell Signaling), NF‐κB p65 (#8242, 1:1000, Cell Signaling), NF‐κB p50 (E‐10; sc‐8414, 1:200, Santa Cruz Biotechnology, CA, USA), B‐cell lymphoma 2 (BCL‐2; sc‐130 307, 1:200, Santa Cruz), glyceraldehyde 3‐phosphate dehydrogenase (GAPDH; sc‐47 724, 1:200, Santa Cruz), BCL‐2 associated X (BAX; K001593, 1:500, Solarbio Life Sciences, Beijing, China), caspase‐3 (sc‐271 028, 1:100, Santa Cruz), poly‐adenosine diphosphate ribose polymerase (PARP; #9542, 1:1000, Cell Signaling), β‐catenin (#9562, 1:1000, Cell Signaling), cMyc (K002749; 1:500, Solarbio Life Sciences), lamin A/C (#4777, 1:2000, Cell Signaling), and β‐actin (K200058M, 1:5000, Solarbio Life Sciences) were treated and stored overnight at 4°C. .. After washing four times for 10 min each with Tris‐buffered saline containing 0.1% Tween‐20 (TBST; pH 7.6), secondary antibodies, horseradish peroxidase (HRP)‐conjugated anti‐rabbit immunoglobulin gamma (IgG; #7074, 1:2000, Cell Signaling) or HRP‐conjugated anti‐mouse IgG (#7076, 1:2000, Cell Signaling), were treated and cultured for 1 h and 30 min, respectively.

Membrane:

Article Title: An Adhesive Peptide Derived from Mussel Protein Alleviates LL37-Induced Rosacea Through Anti-Inflammatory and Anti-Angiogenic Mechanisms
Article Snippet: .. Subsequently, the membrane was incubated overnight at 4 ◦C with primary antibodies against TLR2, KLK5, MMP9, NF-κB (p65), p-NF-κB (p-p65), NF-κB (p50), p-NF-κB (p-p50), IκB-α, p-IκB-α, AKT, p-AKT, ERK1/2, p-ERK1/2, JNK, p-JNK, Involucrin, Claudin-4, Claudin-1, Filaggrin, Occludin, VEGF-A, transforming growth factor beta (TGF-β), PECAM (CD31), and β-actin (Santa Cruz, Dallas, Texas, USA). .. After washing, the membranes were incubated with secondary antibodies for 2 h at room temperature and then treated with the ClarityTM Western ECL substrate (BIO-RAD, Hercules, CA, USA) for 30 s. Images were obtained using the Amersham Imager 680 system (GE Healthcare Life Sciences, Chicago, IL, USA).

Incubation:

other:

Article Title: Physalin A Suppresses Human Oral Squamous Carcinoma Cell Migration and Invasion Through Inhibiting Grb2/Ras and MMP/uPA Signaling Pathways.
Article Snippet: The quantified proteins were electrophoretically separated by 8-12% SDSPAGE gel (based on different KDa), and were transferred to polyvinylidene difluoride (PVDF; Cat. No. IPVH00010; Merck Millipore, Burlington, MA, USA) membranes, which were blocked with 5% albumin.

Article Title: Physalin A Suppresses Human Oral Squamous Carcinoma Cell Migration and Invasion Through Inhibiting Grb2/Ras and MMP/uPA Signaling Pathways
Article Snippet: The membranes were then stained using primary antibodies [anti- plasminogen activator inhibitor 1 (PAI-1; sc-5297; Santa Cruz Biotechnology), -uPA (sc-59727; Santa Cruz Biotechnology), -E-cad (sc-374067; Santa Cruz Biotechnology), -α-tubulin (α-tub; sc-8035; Santa Cruz Biotechnology), -MMP-1 (sc-21731; Santa Cruz Biotechnology), -MMP-2 (sc-13595; Santa Cruz Biotechnology), -MMP-3 (sc-21732; Santa Cruz Biotechnology), -MMP-9 (sc-393859; Santa Cruz Biotechnology), -tissue inhibitor of metalloprotease (TIMP-1; sc-365905; Santa Cruz Biotechnology), -TIMP-2 (sc-21735; Santa Cruz Biotechnology), -p-IKK (p-IKK; sc-293135; Santa Cruz Biotechnology), -IKK (sc-8032; Santa Cruz Biotechnology), -p-IκB (sc-8404; Santa Cruz Biotechnology), -IκB (sc-1643; Santa Cruz Biotechnology), -NF-κB p65 (sc-8008; Santa Cruz Biotechnology), -NF-κB p50 (sc-8414; Santa Cruz Biotechnology), -p-ERK (9911; Cell Signaling Technology), -ERK (9911; Cell Signaling Technology), -p-c-Jun N-terminal kinase (-p-JNK; 4668; Cell Signaling Technology), -JNK (9252; Cell Signaling Technology), -p-p38 (9211; Cell Signaling Technology), -p38 (9212; Cell Signaling Technology), -p-c-Jun (3270; Cell Signaling Technology), -c-Jun (9165; Cell Signaling Technology), -Grb2 (3972; Cell Signaling Technology), -Ras (3965; Cell Signaling Technology), -p-PI3K (4228; Cell Signaling Technology), -PI3K (4292; Cell Signaling Technology), -p-Akt (9271; Cell Signaling Technology), -Akt (9272; Cell Signaling Technology), -PCNA (sc-56; Santa Cruz Biotechnology) and -β-actin (sc-47778; Santa Cruz Biotechnology)] in 4°C overnight.

Review