anti p nf κb p50  (Santa Cruz Biotechnology)

Journal: iScience

Article Title: M2 type tumor-associated macrophage-derived CCL-18 promotes glioma cell proliferation but does not mediates glioma recurrence after radiotherapy

doi: 10.1016/j.isci.2026.117695

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Article Snippet: Anti-p-NF-κB p50(ser337) mouse antibody , Santa Cruz Biotechnology , sc-271908; RRID: AB_10612088.

Techniques: Cell-Signaling, Virus, Tissue, Microarray, Recombinant, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Software, Biomarker Discovery, In Vivo Imaging

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Asiatic acid inhibits NF-κB signaling and ameliorates experimental acute and chronic colitis in mice

doi: 10.1177/03946320251398285

Figure Lengend Snippet: AA inhibits IκBα phosphorylation and degradation in stimulated COLO 205 and RAW 264.7 cells. COLO 205 (a) and RAW 264.7 (b) cells were pretreated with AA (10 or 30 μM) for 24 h or treated with vehicle (0.1% dimethyl sulfoxide [DMSO]). After pretreatment, COLO 205 cells were stimulated with TNF-α (10 ng/mL), and RAW 264.7 cells with LPS (10 μg/mL) for 30 min. Phosphorylated IκBα and IκBα levels were analyzed by Western blotting. β-actin served as a loading control. AA treatment reduced phosphorylated IκBα and preserved IκBα levels, consistent with suppression of nuclear factor-κB (NF-κB) activation. Blots are representative of a single experiment ( n = 1) and were used for qualitative confirmation only. Full-sized, uncropped Western blot images are provided in Supplemental Figure 2 .

Article Snippet: To confirm the specificity of DNA-protein interactions, a supershift assay was performed using anti-NF-κB p50 antibodies (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Phospho-proteomics, Western Blot, Control, Activation Assay

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Asiatic acid inhibits NF-κB signaling and ameliorates experimental acute and chronic colitis in mice

doi: 10.1177/03946320251398285

Figure Lengend Snippet: AA suppresses NF-κB DNA-binding activity in TNF-α-stimulated COLO 205 cells. COLO 205 cells were treated with AA (10 or 30 μM) for 24 h and then stimulated with TNF-α (10 ng/mL) for 30 min. Nuclear extracts were analyzed by electrophoretic mobility shift assay (EMSA) using a biotin-labeled NF-κB consensus probe. AA pretreatment led to a dose-dependent reduction in NF-κB DNA-binding activity. Results shown are representative of n = 2 independent experiments.

Article Snippet: To confirm the specificity of DNA-protein interactions, a supershift assay was performed using anti-NF-κB p50 antibodies (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Binding Assay, Activity Assay, Electrophoretic Mobility Shift Assay, Labeling

Journal: International Journal of Immunopathology and Pharmacology

Article Title: Asiatic acid inhibits NF-κB signaling and ameliorates experimental acute and chronic colitis in mice

doi: 10.1177/03946320251398285

Figure Lengend Snippet: NF-κB p65 immunohistochemistry (IHC) in DSS-induced acute colitis and IL-10⁻/⁻ chronic colitis. (a) Representative IHC images of colon sections from the DSS model (×400 magnification; scale bar = 50 μm). AA-treated mice showed visibly reduced nuclear p65 staining compared with the vehicle group (antibody diluted 1:400). (b) Immunoreactivity score (IRS) for nuclear NF-κB p65 in the DSS model. AA significantly lowered nuclear p65 immunoreactivity compared with the vehicle group. Data are shown as mean ± SD. (c) Representative IHC images from the IL-10⁻/⁻ model (×400 magnification; scale bar = 50 μm). AA treatment decreased nuclear p65 positivity relative to the vehicle group (antibody diluted 1:400). (d) IRS for nuclear NF-κB p65 in the IL-10⁻/⁻ model. AA significantly decreased the immunoreactivity compared to the vehicle group. Data are shown as mean ± SD. DSS and IL-10⁻/⁻: Control, Vehicle, AA ( n = 4/group). Significance: * p < 0.05; ** p < 0.01 (vs vehicle).

Article Snippet: To confirm the specificity of DNA-protein interactions, a supershift assay was performed using anti-NF-κB p50 antibodies (Santa Cruz Biotechnology, Dallas, TX, USA).

Techniques: Immunohistochemistry, Staining, Control